Primate ES Cell Medium

The gold standard culture medium for human ES/iPS cell culture on feeders

  • Jointly developed by ReproCELL and Kyoto University
  • Just add bFGF before use*. No other mixing required.
  • Each lot is functionally tested by using human iPS cells.
  • Lot-to-lot control of other critical criteria including osmolarity, pH, sterility and mycoplasma.
  • Serum-free
The trusted culture medium for human ESC/iPSC culture on feeders
 

ReproCELL’s Primate ES Cell Medium is a serum-free formulation for feeder-dependent ES (embryonic stem) / iPS (induced pluripotent stem) cell culture that was jointly developed by ReproCELL and Kyoto University. This stem cell media enabled development of the world’s first human induced pluripotent stem cells in 2007 by Prof Shinya Yamanaka and his colleagues. Following this breakthrough, ReproCELL’s Primate ES Cell Culture Medium has been successfully adopted for culture of stem cells by numerous labs throughout the world to promote a wide range of stem cell applications. This stem cell media has been specifically developed to simplify the process of ES and iPS cell culture for human and other primate cells. You just need to add bFGF prior to use* and the medium is ready for use.

For related culture reagents to culture, dissociate, passage and cryopreserve the stem cells, please see the bottom of the page.

*: not necessary for all the cell lines

human iPSC cultured using Primate ES Cell Medium with 5ng/ml bFGF on MEF

The instruction manual for Primate ES Cell Medium (PDF, open in a new window)
Instruction Movie for human iPS cell culture (Jump to the Movie, open in a new window)

 

[Movie] human iPSC growing in Primate ES Cell Medium

 

Publications

A list of published studies in which Primate ES Cell Medium was used, which include the monumental study performed by Prof. Shinya Yamanaka, is here.

 

Product list
Catalog No. Product Retail Price Quantity Purchase
RCHEMD001 Primate ES Cell Medium (500mL) $210.00

Contact the distributor of your region
RCHEMD001A Primate ES Cell Medium (500mL) X 5 pack $945.00

Contact the distributor of your region
RCHEMD001B Primate ES Cell Medium (500mL) X 5 & bFGF (25 ug) pack $995.00

Contact the distributor of your region
Payment method can be chosen from Paypal / credit card / bank transfer.

 

Data Sheets

The data sheet for each lot of the product (open in a new window)

 

  • Human ES/iPS Cell Culture Kit: Culture kit for feeder-dependent culture of human ES/iPS cells
  • ReproCoat: Coating solution for feeder-dependent culture of human ES/iPS cells. Works well with feeder cells including MEF, SL10 and SNL.
  • ReproFF2: A new feeder-free culture medium for human ES/iPS cells
  • Freezing Medium: For cryopreservation of human ES/iPS cells
  • Dissociation Solution: For gentle dissociation and passage of human ES/iPS the cells (ctk solution)
  • bFGF: human recombinant fgf-2.
  • anti-human nanog antibody: For monitoring the pluripotency maintenance